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Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes
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JoVE Journal 遺伝学
Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

DOI:
10.3791/59988-v

•

09:12 min

•

December 20, 2019

•

, , , , , ,
1Laboratory of Biology, BIO@SNS,Scuola Normale Superiore

章

  • 00:05標題
  • 00:32Cell Transfection
  • 01:46Western Blot Sample Extraction
  • 05:00Western Blot
  • 06:51Results: Representative Tau Subcellular Enrichment Analyses
  • 08:39Conclusion

概要

自動翻訳

自動翻訳

Tau is a neuronal protein present both in the cytoplasm, where it binds microtubules, and in the nucleus, where it exerts unconventional functions including the modulation of Alzheimer's disease-related genes. Here, we describe a method to investigate the function of nuclear Tau while excluding any interferences coming from cytoplasmic Tau.

タグ

TauSubcellular LocalizationNuclear FunctionSH-SY5YTransfectionTau-NLSTau-NESWestern BlotProtein ExtractionLysis Buffer

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