Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

Masamune Morita; Yuri Ota; Kaoru Katoh; Naohiro Noda

doi:10.3791/59555

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免疫学と感染

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Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

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免疫学と感染

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JoVE Journal 免疫学と感染

Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

6,723 Views

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07:33 min

•

April 30, 2019

DOI:

10.3791/59555-v

DOI:

10.3791/59555-v

•

07:33 min

April 30, 2019

•

1 Views

Masamune Morita¹, Yuri Ota^1,2, Kaoru Katoh¹, Naohiro Noda^1,2

¹Biomedical Research Institute,National Institute of Advanced Industrial Science and Technology (AIST), ²Department of Life Science and Medical Bioscience,Waseda University

概要

We demonstrate single-cell culture of bacteria inside giant vesicles (GVs). GVs containing bacterial cells were prepared by the droplet transfer method and were immobilized on a supported membrane on a glass substrate for direct observation of bacterial growth. This approach may also be adaptable to other cells.

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Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles

概要

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