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Culturing and Measuring Fetal and Newborn Murine Long Bones
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JoVE Journal 発生生物学
Culturing and Measuring Fetal and Newborn Murine Long Bones

Culturing and Measuring Fetal and Newborn Murine Long Bones

DOI:
10.3791/59509-v

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06:58 min

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April 26, 2019

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,
1Australian Regenerative Medicine Institute,Monash University

章

  • 00:04標題
  • 00:42Fetal Tibia and Femur Culture and Full Bone Length and Mineralized Region Analysis
  • 04:50Results: Representative Total Bone Length Growth and Mineralized Tibia Measurement and Analysis
  • 06:24Conclusion

概要

自動翻訳

自動翻訳

Here, we present a method for ex vivo culture of long murine bones at both fetal and newborn stages, suitable for analyzing bone and cartilage development and homeostasis in controlled conditions while recapitulating the in vivo process.

タグ

FetalNewbornMurineLong BonesMetatarsal CultureBone DevelopmentEx Vivo CultureGenetic ModelsLive ImagingPhysical ManipulationPharmacological ManipulationDissectionLimb SeparationBone Culture

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