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Identificatie van Orexin en endocannabinoïde receptoren bij volwassen zebravis met behulp van immunoperoxidasekleuring en immunofluorescentie methoden
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生化学
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JoVE Journal 生化学
Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
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Identificatie van Orexin en endocannabinoïde receptoren bij volwassen zebravis met behulp van immunoperoxidasekleuring en immunofluorescentie methoden

7,820 Views

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08:37 min

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June 25, 2019

DOI:

10.3791/59308-v

DOI:
10.3791/59308-v

•

08:37 min
June 25, 2019

•

1 Views
, , , ,
1Department of Sciences and Technologies (DST),University of Sannio, 2Endocannabinoid Research Group,Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions,University of Naples Federico II

概要

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Hier gepresenteerd zijn protocollen voor immunohistochemische karakterisering en lokalisatie van orexin peptide, orexin receptoren, en endocannabinoïde receptoren in de darm en hersenen van normale en dieet-geïnduceerde obesitas (DIO) Adult zebravis modellen met behulp van immunoperixidase en dubbele immunofluorescentie methodes.

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Cite this Article

Imperatore, R., D'Angelo, L., De Girolamo, P., Cristino, L., Paolucci, M. Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods. J. Vis. Exp. (148), e59308, doi:10.3791/59308 (2019).

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