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使用免疫过氧化物酶和免疫荧光方法鉴定成年斑马鱼中的奥列辛和内他大麻素受体
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JoVE Journal 生化学
Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
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使用免疫过氧化物酶和免疫荧光方法鉴定成年斑马鱼中的奥列辛和内他大麻素受体

7,820 Views

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08:37 min

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June 25, 2019

DOI:

10.3791/59308-v

DOI:
10.3791/59308-v

•

08:37 min
June 25, 2019

•

1 Views
, , , ,
1Department of Sciences and Technologies (DST),University of Sannio, 2Endocannabinoid Research Group,Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions,University of Naples Federico II

概要

Automatically generated

这里介绍的是免疫组织化学表征和定位的orexin肽,orexin受体,和内分泌大麻素受体在肠道和大脑中正常和饮食诱发肥胖(DIO)成年斑马鱼模型使用免疫渗透酶和双免疫荧光法。

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Cite this Article

Imperatore, R., D'Angelo, L., De Girolamo, P., Cristino, L., Paolucci, M. Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods. J. Vis. Exp. (148), e59308, doi:10.3791/59308 (2019).

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