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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
JoVE Journal
遺伝学
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JoVE Journal 遺伝学
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

8,689 Views

13:04 min

March 01, 2019

DOI:

13:04 min
March 01, 2019

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概要

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Traditional cDNA-based overexpression techniques have a limited applicability for the overexpression of long noncoding RNAs due to their multiple splice forms with potential functionality. This review reports a protocol using CRISPR technology to overexpress multiple splice variants of a long noncoding RNA.

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