このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
8,694 Views
•
13:04 min
•
March 01, 2019
DOI:
1Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine,University of California, Los Angeles, 2Vatche and Tamar Manoukian Division of Digestive Diseases, Integrated Molecular Technologies (IMT) Core, Department of Medicine,University of California, Los Angeles, 3Division of Hematology and Oncology, Department of Medicine,University of California, Los Angeles
概要
Automatically generated
Traditional cDNA-based overexpression techniques have a limited applicability for the overexpression of long noncoding RNAs due to their multiple splice forms with potential functionality. This review reports a protocol using CRISPR technology to overexpress multiple splice variants of a long noncoding RNA.
Read Article
Cite this Article
Rankin, C. R., Treger, J., Faure-Kumar, E., Benhammou, J., Anisman-Posner, D., Bollinger, A. E., Pothoulakis, C., Padua, D. M. Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR. J. Vis. Exp. (145), e59233, doi:10.3791/59233 (2019).
Copy