Traditional cDNA-based overexpression techniques have a limited applicability for the overexpression of long noncoding RNAs due to their multiple splice forms with potential functionality. This review reports a protocol using CRISPR technology to overexpress multiple splice variants of a long noncoding RNA.
Rankin, C. R., Treger, J., Faure-Kumar, E., Benhammou, J., Anisman-Posner, D., Bollinger, A. E., Pothoulakis, C., Padua, D. M. Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR. J. Vis. Exp. (145), e59233, doi:10.3791/59233 (2019).