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Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro
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March 18, 2019
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This protocol includes dissection of murine long bones and bone marrow isolation, to generate bone marrow macrophages and produce osteoclasts using macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor Kappa-B ligand (RANKL) or tumor necrosis factor alpha (TNF-α) and their arrest by anti-c-fms antibody, M-CSF receptor.
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Marahleh, A., Kitaura, H., Ishida, M., Shima, K., Kishikawa, A., Ogawa, S., Shen, W., Qi, J., Ohori, F., Noguchi, T., Nara, Y., Mizoguchi, I. Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro. J. Vis. Exp. (145), e59089, doi:10.3791/59089 (2019).
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