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Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction
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JoVE Journal 化学
Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction

Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction

DOI:
10.3791/59041-v

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09:44 min

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January 29, 2019

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, ,
1化学科,Hunter College of the City University of New York, 2Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 3Department of Radiology,Memorial Sloan Kettering Cancer Center, 4Department of Radiology,Weill Cornell Medical College

章

  • 00:04標題
  • 00:41The Preparation of huA33-TCO
  • 03:54177Lu Radiolabeling of Tz-PEG7-DOTA
  • 05:10In vivo Studies
  • 07:08Results: Longitudinal Therapy Study of Mice Bearing Subcutaneous SW1222 Tumors
  • 09:00Conclusion

概要

自動翻訳

自動翻訳

This protocol describes the synthesis and characterization of a trans-cyclooctene (TCO)-modified antibody and a 177Lu-labeled tetrazine (Tz) radioligand for pretargeted radioimmunotherapy (PRIT). In addition, it details the use of these two constructs for in vivo biodistribution and longitudinal therapy studies in a murine model of colorectal cancer.

タグ

Pretargeted RadioimmunotherapyPRITInverse Electron Demand Diels-Alder ReactionColorectal Cancer XenograftAntibody ConjugationTCO-NHSHuA33 AntibodySize Exclusion ChromatographyUV-vis Spectrophotometry

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