This content is Free Access.
How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
27,290 Views
•
07:22 min
•
February 11, 2019
DOI:
概要
Automatically generated
A protocol is presented to rapidly test the thermal stability of proteins in a variety of conditions through thermal shift assays and nano differential scanning fluorimetry. Buffer systems, salts and additives, together comprising three unique stability screens, are assayed with proteins to identify suitable buffers for functional and structural studies.
Read Article
Cite this Article
Bruce, D., Cardew, E., Freitag-Pohl, S., Pohl, E. How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project. J. Vis. Exp. (144), e58666, doi:10.3791/58666 (2019).
Copy