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Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

DOI:
10.3791/58274-v

•

07:50 min

•

August 29, 2018

•

, , ,
1BioTime, Inc

章

  • 00:04標題
  • 00:59Tissue Preparation for Immunostaining
  • 03:02Immunostaining with 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) Antibodies
  • 05:02Results: Immunostaining with 5mC and 5hmC to Determine Their Distribution During Retinal Development
  • 06:49Conclusion

概要

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自動翻訳

The objective of this report is to describe the protocol for robust immunohistochemical detection of epigenetic markers, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in developing and postmitotic mouse retina.

タグ

Immunohistochemistry5-methylcytosine5-hydroxymethylcytosineMouse RetinaRetinal DevelopmentChromatinFrozen Histological PreparationCryosectioningImmunostainingDNA Methylation

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