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基于寡核苷酸的串联 RNA 分离法恢复真核 mRNA-蛋白复合物
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生化学
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JoVE Journal 生化学
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
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基于寡核苷酸的串联 RNA 分离法恢复真核 mRNA-蛋白复合物

10,688 Views

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09:45 min

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August 18, 2018

DOI:

10.3791/58223-v

DOI:
10.3791/58223-v

•

09:45 min
August 18, 2018

•

6 Views
, ,
1Dept. of Microbial Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences,University of Surrey

概要

Automatically generated

本文介绍了一种用于恢复内在形成的 mRNA 蛋白复合物的串联 RNA 分离过程 (跳闸)。具体来说, rna 蛋白复合物在体内交联, polyadenylated rna 与寡核苷酸 (dT) 珠提取物分离, 特殊基因用修饰 RNA 反义寡核苷酸捕获。通过应用免疫印迹分析, 检测到基因的蛋白质。

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Cite this Article

Iadevaia, V., Matia-González, A. M., Gerber, A. P. An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes. J. Vis. Exp. (138), e58223, doi:10.3791/58223 (2018).

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