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微穿刺2光子显微镜辅助小鼠鲍曼空间的研究
JoVE Journal
生物学
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JoVE Journal 生物学
Micropuncture of Bowman’s Space in Mice Facilitated by 2 Photon Microscopy
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微穿刺2光子显微镜辅助小鼠鲍曼空间的研究

9,008 Views

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07:37 min

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October 11, 2018

DOI:

10.3791/58206-v

DOI:
10.3791/58206-v

•

07:37 min
October 11, 2018

•

7 Views
, , , , , ,
1Anesthesiology & Perioperative Medicine,Oregon Health & Science University, 2Environmental and Biological Services Division,Pacific Northwest National Laboratory, 3Operative Care Division,Portland Veterans Affairs Medical Center

概要

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我们目前使用2光子显微术在鲍曼的小鼠泌尿空间内放置一个微量吸管, 结合2个肾脏生理学的基础技术。使用2光子显微术克服了传统显微术微穿刺肾脏生理学研究的关键局限性。

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Cite this Article

Matsushita, K., Golgotiu, K., Orton, D. J., Smith, R. D., Rodland, K. D., Piehowski, P. D., Hutchens, M. P. Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy. J. Vis. Exp. (140), e58206, doi:10.3791/58206 (2018).

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