ジャーナル
/
発生生物学
/
Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo

Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo

DOI:
10.3791/57900-v

•

08:23 min

•

November 02, 2018

•

, , ,
1CNRS, Institut de Biologie du Développement de Marseille,Aix-Marseille Université

章

  • 00:04標題
  • 00:49Preparing the Optical Tweezers
  • 02:01Interfacing the Instrument
  • 02:52Calibration of the Optical Trap
  • 03:45Position Interpolation with Image Analysis
  • 04:30Mounting Drosophila Embryos
  • 05:12Trapping Experiment In Vivo
  • 06:06Mechanical Measurements
  • 06:44Results: Interface Deflection Imposed by Sinusoidal Movement
  • 07:50Conclusion

概要

自動翻訳

自動翻訳

We present a setup of optical tweezers coupled to a light sheet microscope, and its implementation to probe cell mechanics without beads in the Drosophila embryo.

タグ

Optical TweezersDrosophila EmbryoCell-cell ContactsLive ImagingLight Sheet MicroscopyFluorescent BeadsLaserQT CreatorMicroRheoData Acquisition BoardOptical Shutter Interface

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Microbead Implantation in the Zebrafish Embryo

Imaging Subcellular Structures in the Living Zebrafish Embryo

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

AFM and Microrheology in the Zebrafish Embryo Yolk Cell

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo

Live Imaging and Analysis of Muscle Contractions in Drosophila Embryo

Read Article