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Trattamento combinatorio di tricostatina A e la vitamina C migliora l'efficienza di clonazione topi da trasferimento nucleare di cellule somatiche
1Division of Biological Science, Graduate School of Biology-Oriented Science and Technology,Kindai University, 2Faculty of Biology-Oriented Science and Technology,Kindai University, 3Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology,University of Cambridge, 4Laboratory of Reproductive Biology, Graduate School of Agriculture,Kyoto University, 5Institute of Advanced Technology,Kindai University
章
- 00:05標題
- 00:52Preparation of Deionized Bovine Serum Albumin
- 01:48Oocyte Collection
- 03:26Preparation of Donor Cells for Nuclear Transfer and Enucleation of Oocytes
- 05:32Fusion of Donor Cell and Enucleated Oocyte
- 07:42Activation of Reconstructed Oocytes and Treatment with Trichostatin A and Vitamin C
- 08:26Results: Somatic Cell Nuclear Transfer in Mice using Trichostatin A and Vitamin C Treatment
- 09:15Conclusion
Descriviamo un metodo migliorato notevolmente per mouse clonazione usando tricostatina A, vitamina C e deionizzata albumina di siero bovino. Vi mostriamo un protocollo semplificato, riproducibile che supporta lo sviluppo efficiente di embrioni clonati. Quindi, questo metodo potrebbe diventare una procedura standardizzata per la clonazione del mouse.
