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用生物层干涉法捕获小分子离子通道蛋白的相互作用动力学
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Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay
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用生物层干涉法捕获小分子离子通道蛋白的相互作用动力学

8,028 Views

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10:41 min

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March 07, 2018

DOI:

10.3791/56846-v

DOI:
10.3791/56846-v

•

10:41 min
March 07, 2018

•

3 Views
, , ,
1Department of General Surgery, Tongren Hospital,Shanghai Jiao Tong University School of Medicine, 2Key Laboratory of Systems Biomedicine (Ministry of Education), Institute of Systems Biomedicine,Shanghai Jiao Tong University, 3Hongqiao International Institute of Medicine, Tongren Hospital,Shanghai Jiao Tong University School of Medicine

概要

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该协议描述了纯化 hEAG1 离子通道蛋白与小分子脂配体磷酸肌醇4、5-bisphosphate (PIP2) 的相互作用。实验表明, BLI 可能是一种新的小分子离子通道配体筛选的潜在方法。

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Bio-layer InterferometryBLI AssayIon ChannelProtein-small Molecule InteractionKineticsHEAG1Cell CultureProtein ExpressionPurificationAffinity Chromatography

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Cite this Article

Han, B., Zhang, M., Sun, P., Hou, S. Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay. J. Vis. Exp. (133), e56846, doi:10.3791/56846 (2018).

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