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粘附细胞培养的时间推移荧光显微镜对蛋白质降解率的单细胞定量研究
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発生生物学
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JoVE Journal 発生生物学
Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture
Please note that all translations are automatically generated. Click here for the English version.

粘附细胞培养的时间推移荧光显微镜对蛋白质降解率的单细胞定量研究

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12:06 min

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February 04, 2018

DOI:

10.3791/56604-v

DOI:
10.3791/56604-v

•

12:06 min
February 04, 2018

•

7 Views
,
1UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences,École Polytechnique Fédérale de Lausanne (EPFL)

概要

Automatically generated

该协议描述了一种方法, 以确定蛋白质半生命的单一生活粘附细胞, 使用脉冲标记和荧光时间推移成像的捕捉标签融合蛋白。

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Cite this Article

Alber, A. B., Suter, D. M. Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture. J. Vis. Exp. (132), e56604, doi:10.3791/56604 (2018).

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