Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture

Andrea Brigitta Alber; David Michael Suter

doi:10.3791/56604

/

Cella singola quantificazione dei tassi di degradazione delle proteine mediante microscopia a fluorescenza time-lapse in coltura delle cellule aderenti

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発生生物学
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JoVE Journal 発生生物学

Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture

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Cella singola quantificazione dei tassi di degradazione delle proteine mediante microscopia a fluorescenza time-lapse in coltura delle cellule aderenti

8,460 Views

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12:06 min

•

February 04, 2018

DOI:

10.3791/56604-v

DOI:

10.3791/56604-v

•

12:06 min

February 04, 2018

•

7 Views

Andrea Brigitta Alber¹, David Michael Suter¹

¹UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences,École Polytechnique Fédérale de Lausanne (EPFL)

概要

Questo protocollo descrive un metodo per determinare la proteina emivite in singole cellule aderenti, utilizzando impulsi etichettatura e time-lapse imaging di fluorescenza di proteine di fusione SNAP-tag.

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Protein Degradation Single-cell Time-lapse Fluorescence Microscopy Adherent Cell Culture SNAP-tag Protein Half-life Fluorescence Imaging Protein Turnover Heterogeneity Endogenous Protein Tagging Overexpression DNA Repair Enzyme Benzylguanine Fluorescent Dye Exponential Decay Embryonic Stem Cells Monolayer Cell-cell Interactions N-cadherin

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Cella singola quantificazione dei tassi di degradazione delle proteine mediante microscopia a fluorescenza time-lapse in coltura delle cellule aderenti

概要

Automatically generated

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