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Eencellige kwantificering van eiwit afbraak tarieven door Time-Lapse fluorescentie microscopie in aanhangend celkweek
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JoVE Journal 発生生物学
Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture
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Eencellige kwantificering van eiwit afbraak tarieven door Time-Lapse fluorescentie microscopie in aanhangend celkweek

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12:06 min

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February 04, 2018

DOI:

10.3791/56604-v

DOI:
10.3791/56604-v

•

12:06 min
February 04, 2018

•

7 Views
,
1UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences,École Polytechnique Fédérale de Lausanne (EPFL)

概要

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Dit protocol beschrijft een methode om te bepalen eiwit halfwaardetijd in één levende Adherente cellen, met behulp van pulse labeling en fluorescentie time-lapse beeldvorming van module-tag fusion eiwitten.

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Cite this Article

Alber, A. B., Suter, D. M. Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture. J. Vis. Exp. (132), e56604, doi:10.3791/56604 (2018).

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