ジャーナル
/
がん研究
/
单细胞下游特征的靶细胞预富集与全基因组扩增
JoVE Journal
がん研究
This content is Free Access.
JoVE Journal がん研究
Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
Please note that all translations are automatically generated. Click here for the English version.

单细胞下游特征的靶细胞预富集与全基因组扩增

8,584 Views

•

10:12 min

•

May 15, 2018

DOI:

10.3791/56394-v

DOI:
10.3791/56394-v

•

10:12 min
May 15, 2018

•

3 Views
, , , , , , , ,
1Institute of Cell Biology, Histology and Embryology,Medical University of Graz, 2Institute of Pathology,Medical University of Graz, 3Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, 4Department of Tumor Biology,University Medical Center Hamburg-Eppendorf, 5Sahlgrenska Cancer Center,University of Gothenburg

概要

Automatically generated

该协议是从非目标背景细胞的混合物中恢复和制备稀有靶细胞, 用于单细胞水平的分子遗传表征。DNA 质量等于未处理的单个细胞, 并允许单细胞应用 (筛查和靶向分析)。

Explore More Videos

Single CellCell SuspensionLiquid BiopsyCellular HeterogeneityCirculating Tumor CellsCell CharacterizationCell IsolationCell HarvestingWhole Genome AmplificationPre-enrichmentHoechst 33342CFSE LabelingHemocytometerFluorescence MicroscopyCell CountingCell ResuspensionPeripheral Blood

Read Article

Cite this Article

Chen, S., El-Heliebi, A., Schmid, J., Kashofer, K., Czyż, Z. T., Polzer, B. M., Pantel, K., Kroneis, T., Sedlmayr, P. Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization. J. Vis. Exp. (135), e56394, doi:10.3791/56394 (2018).

Download .ris file

Copy

Share Video

.

Copy