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水平整体安装：厚三维组织横截面皮肤的新型加工和成像方案
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin
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水平整体安装：厚三维组织横截面皮肤的新型加工和成像方案

14,515 Views

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08:31 min

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August 02, 2017

DOI:

10.3791/56106-v

DOI:
10.3791/56106-v

•

08:31 min
August 02, 2017

•

9 Views
,
1Centre for Stem Cells and Regenerative Medicine,King’s College London, 2School of Molecular Biosciences,Washington State University

概要

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这项工作提出了一种新颖的加工和成像协议，用于厚的三维组织横截面分析，可以充分利用共聚焦成像模式。该协议保留抗原性，并代表一个强大的系统来分析皮肤组织学和潜在的其他组织类型。

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Cite this Article

Salz, L., Driskell, R. R. Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin. J. Vis. Exp. (126), e56106, doi:10.3791/56106 (2017).

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