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The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging
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JoVE Journal 発生生物学
The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging

The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging

9,447 Views

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10:30 min

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October 03, 2017

DOI:

10.3791/56101-v

DOI:
10.3791/56101-v

•

10:30 min
October 03, 2017

•

1 Views
, , , , ,
1Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital for Children,Harvard Medical School, 2College of Life Sciences,Jilin University, 3Faculty of Health Sciences,University of Macau

概要

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The C. elegans excretory canal is a unique single-cell model for the visual in vivo analysis of de novo polarized membrane biogenesis. This protocol describes a combination of standard genetic/RNAi and imaging approaches, adaptable for the identification and characterization of molecules directing unicellular tubulogenesis, and apical membrane and lumen biogenesis.

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C. ElegansExcretory CanalIntracellular LumenPolarized Membrane BiogenesisTubulogenesisPolarityGFP FusionRNAiLive ImagingMembrane MarkersOrganelle MarkersPromotersERM-1Cystic Canal PhenotypeModifier Screen

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Cite this Article

Zhang, N., Membreno, E., Raj, S., Zhang, H., Khan, L. A., Gobel, V. The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging. J. Vis. Exp. (128), e56101, doi:10.3791/56101 (2017).

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