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Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space
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JoVE Journal 神経科学
Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space

Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space

DOI:
10.3791/55755-v

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10:45 min

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July 24, 2017

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, , , , ,
1医学部,University of Virginia, 2Department of Cell Biology,SUNY Downstate Medical Center, 3Neural and Behavioral Science Graduate Program,SUNY Downstate Medical Center, 4Division of Neonatology,University of Virginia, 5Department of Neuroscience and Physiology,New York University School of Medicine

章

  • 00:05標題
  • 01:01Real-time Iontophoresis in Agarose
  • 03:59Agarose Data Analysis
  • 05:30Real-time Iontophoresis in Brain Slices
  • 07:02Brain Data Analysis, Checking Transport Number and ISM Calibration
  • 08:19Results: Curve Fitting Data in Agar and in Brain
  • 09:21Conclusion

概要

自動翻訳

自動翻訳

This protocol describes real-time iontophoresis, a method that measures physical parameters of the extracellular space (ECS) of living brains. The diffusion of an inert molecule released into the ECS is used to calculate the ECS volume fraction and tortuosity. It is ideal for studying acute reversible changes to brain ECS.

タグ

IontophoresisTetramethylammoniumExtracellular SpaceVolume FractionTortuosityACSFTMAISMWandaWalterMicromanipulatorCalibrationTemperature

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