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Ex 活体人肺肿瘤切片中居民 CD8 T 淋巴细胞的共聚焦显微成像
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December 27, 2017
DOI:
1Institut Cochin, Inserm U1016-CNRS UMR8104,Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals,Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center,University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals,University Paris Descartes
概要
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本协议描述了一种图像驻留肿瘤浸润 CD8 T 细胞标记的人肺肿瘤切片荧光耦合抗体的方法。这项技术允许 real-time 分析 CD8 T 细胞迁移使用共聚焦显微镜。
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Cite this Article
Peranzoni, E., Bougherara, H., Barrin, S., Mansuet-Lupo, A., Alifano, M., Damotte, D., Donnadieu, E. Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy. J. Vis. Exp. (130), e55709, doi:10.3791/55709 (2017).
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