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Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

DOI:

06:05 min

May 25, 2017

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  • 00:05標題
  • 00:38Human Plasma Acetylated Low Density Lipoprotein Dil Complex (Dil-AcLDL) Injection Preparation
  • 02:27Dil-AcLDL Injection and Xenopus tropicalis Embryo Fixation
  • 03:44Results: Representative Xenopus tropicalis Imaging
  • 05:02Conclusion

概要

自動翻訳

This protocol demonstrates a fluorescence-based method to visualize the vasculature and to quantify its complexity in Xenopus tropicalis. Blood vessels can be imaged minutes after the injection of a fluorescent dye into the beating heart of an embryo after genetic and/or pharmacological manipulations to study cardiovascular development in vivo.

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