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多层皮质Ca<sup> 2+</sup>使用棱镜探针和小型荧光显微镜成像自由移动的小鼠
JoVE Journal
行動学
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JoVE Journal 行動学
Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy
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多层皮质Ca<sup> 2+</sup>使用棱镜探针和小型荧光显微镜成像自由移动的小鼠

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10:35 min

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June 13, 2017

DOI:

10.3791/55579-v

DOI:
10.3791/55579-v

•

10:35 min
June 13, 2017

•

16 Views
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1Inscopix Inc.

概要

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在这里，我们提出了一种在自由移动的小鼠中通过多个皮质层进行细胞分辨率的大规模Ca 2+成像的程序。可以使用与植入式棱镜探头耦合的微型头戴式显微镜同时观察数百个活性细胞。

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Cortical Calcium ImagingPrism ProbesMiniaturized Fluorescence MicroscopyFreely Moving MiceGenetically Encoded NeuronsMulti-layer Cortical ActivityNeural Circuit FunctionSystems Neuroscience

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Cite this Article

Gulati, S., Cao, V. Y., Otte, S. Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy. J. Vis. Exp. (124), e55579, doi:10.3791/55579 (2017).

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