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단백질 인산 가수분해 효소 2A 촉매 소 단위 셀 무료 분석 실험에 활동을 자극 하는 재조합 α-β-및 γ-Synucleins
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神経科学
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JoVE Journal 神経科学
Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays
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단백질 인산 가수분해 효소 2A 촉매 소 단위 셀 무료 분석 실험에 활동을 자극 하는 재조합 α-β-및 γ-Synucleins

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09:36 min

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August 13, 2017

DOI:

10.3791/55361-v

DOI:
10.3791/55361-v

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09:36 min
August 13, 2017

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1Department of Biomedical Sciences, Center of Emphasis in Neurosciences, Graduate School of Biomedical Sciences, Paul L. Foster School of Medicine,Texas Tech University Health Sciences Center El Paso

タグ

Recombinant SynucleinProtein Phosphatase 2ACell-free AssayP-Nitrophenol Phosphate (pNPP) BufferMalachite Green SolutionTween 20 ActivatorThreonine PhosphopeptidePhosphate Standards

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Lek, S., Vargas-Medrano, J., Villanueva, E., Marcus, B. S., Godfrey, W., Perez, R. G. Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays. J. Vis. Exp. (126), e55361, doi:10.3791/55361 (2017).

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