Presented here is a simple technique for high-resolution confocal time-lapse imaging of root and hypocotyl development for up to 3 days using high numerical-aperture objectives and perfluorodecalin as an immersion medium.
Kirchhelle, C., Moore, I. A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development. J. Vis. Exp. (123), e55331, doi:10.3791/55331 (2017).