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라이브 영상에 대한 성숙한 근섬유의 체외 분화
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
In Vitro Differentiation of Mature Myofibers for Live Imaging

라이브 영상에 대한 성숙한 근섬유의 체외 분화

DOI:

08:12 min

January 07, 2017

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  • 00:05標題
  • 00:46Neonatal Mouse Hind-limb Dissection
  • 02:09Myoblast Isolation
  • 04:09Myofiber Differentiation and Immunostaining
  • 06:10Results: Developmental Stages of the Myoblast Culture
  • 07:21Conclusion

概要

自動翻訳

Muscle cells are among the most complex eukaryotic cells. We present a protocol for the in vitro differentiation of highly mature myofibers that allows for genetic manipulation and clear imaging during all developmental stages.

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