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Ein Verfahren zur Messung des Metabolismus in sortierter Subpopulationen von komplexen Zell Gemeinschaften Verwendung stabiler Isotopen-Tracing
JoVE Journal
生化学
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JoVE Journal 生化学
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

Ein Verfahren zur Messung des Metabolismus in sortierter Subpopulationen von komplexen Zell Gemeinschaften Verwendung stabiler Isotopen-Tracing

DOI:

07:41 min

February 04, 2017

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  • 00:05標題
  • 00:52Metabolite Extraction from a Dish
  • 01:53Metabolite Extraction of Sorted Cell
  • 04:26Data Analysis
  • 05:22Results: Representative Mas Isotopomer Distribution Validation and Cell Cycle Phase Difference
  • 06:50Conclusion

概要

自動翻訳

This article describes a method for studying cellular metabolism in complex communities of multiple cell types, using a combination of stable isotope tracing, cell sorting to isolate specific cell types, and mass spectrometry.

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