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마우스 마음에서 과발현하는 rAAV9의 제조 또는 최저 유전자
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遺伝学
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JoVE Journal 遺伝学
Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts

마우스 마음에서 과발현하는 rAAV9의 제조 또는 최저 유전자

DOI:

11:11 min

December 17, 2016

, , , , , ,

  • 00:05標題
  • 00:57Transfection of HEK293 with rAAV9 Plasmids
  • 02:14Harvest of Transfected HEK293 Cells and Purification of rAAV9 Vectors
  • 07:04rAAV9 Injections into Neonatal Mice
  • 08:10Results: rAAV9 Overexpression and Gene Knockdown in Mouse Hearts
  • 10:18Conclusion

概要

自動翻訳

In this manuscript, a method to prepare recombinant adeno-associated virus 9 (rAAV9) vectors to manipulate gene expression in the mouse heart is described.

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