A detailed protocol describing the SSTD NMR method is presented here to help new users apply this new method to obtain the kinetic parameters of their own systems undergoing chemical exchange.
Quirós, M. T., Macdonald, C., Angulo, J., Muñoz, M. P. Spin Saturation Transfer Difference NMR (SSTD NMR): A New Tool to Obtain Kinetic Parameters of Chemical Exchange Processes. J. Vis. Exp. (117), e54499, doi:10.3791/54499 (2016).