This content is Free Access.
Approcci genetici e biochimici per<em> In Vivo</em> e<em> In Vitro</em> La valutazione di proteine Oligomerizzazione: Il rianodina Receptor Case Study
11,113 Views
•
12:43 min
•
July 27, 2016
DOI:
概要
Automatically generated
Oligomerization of the ryanodine receptor, a homo-tetrameric ion channel mediating Ca2+ release from intracellular stores, is critical for skeletal and cardiac muscle contraction. Here, we present complementary in vivo and in vitro methods to detect protein self-association and determine homo-oligomer stoichiometry.
Explore More Videos
Protein OligomerizationRyanodine ReceptorYeast Two-hybridCo-immunoprecipitationChemical Cross-linkingProtein-protein InteractionsHomo-oligomersCardiopathiesCardiac ArrhythmiasSudden DeathAnti-arrhythmic DrugsCompetent Yeast CellsPlasmid DNALithium AcetatePEG SolutionDimethyl SulfoxideMinimal SD Medium Plates
Read Article
Cite this Article
Stanczyk, P. J., Lai, F. A., Zissimopoulos, S. Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study. J. Vis. Exp. (113), e54271, doi:10.3791/54271 (2016).
Copy