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Genetische en biochemische benaderingen voor<em> In Vivo</em> en<em> In Vitro</em> Evaluatie van Protein Oligomerisatie: De ryanodinereceptor Case Study
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July 27, 2016
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Oligomerization of the ryanodine receptor, a homo-tetrameric ion channel mediating Ca2+ release from intracellular stores, is critical for skeletal and cardiac muscle contraction. Here, we present complementary in vivo and in vitro methods to detect protein self-association and determine homo-oligomer stoichiometry.
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Stanczyk, P. J., Lai, F. A., Zissimopoulos, S. Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study. J. Vis. Exp. (113), e54271, doi:10.3791/54271 (2016).
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