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腓骨神経損傷方法：神経筋接合部を修復信頼性の高い同定するためのアッセイおよびテスト要因
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神経科学
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JoVE Journal 神経科学
The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions
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腓骨神経損傷方法：神経筋接合部を修復信頼性の高い同定するためのアッセイおよびテスト要因

15,264 Views

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06:01 min

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August 11, 2016

DOI:

10.3791/54186-v

DOI:
10.3791/54186-v

•

06:01 min
August 11, 2016

•

10 Views
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1Carilion Research Institute,Virginia Tech, 2Carilion School of Medicine,Virginia Tech, 3Department of Biological Sciences,Virginia Tech

概要

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We have developed a nerve injury method to reliably examine muscle reinnervation, and thus regeneration of neuromuscular junctions in mice. This technique involves injuring the common fibular nerve via a simple and highly reproducible surgery. Muscle reinnervation in then assessed by whole-mounting the extensor digitorum longus muscle.

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Keyword Extraction:Fibular Nerve InjuryNeuromuscular JunctionsNerve RegenerationPeripheral Nerve InjuryNerve CrushNerve Injury MethodMouse ModelSurgical ProcedureNerve TracingMuscle SeparationNerve ExposureNerve Crush Injury

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Cite this Article

Dalkin, W., Taetzsch, T., Valdez, G. The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions. J. Vis. Exp. (114), e54186, doi:10.3791/54186 (2016).

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