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עצב שׁוֹקִיתִי השיטה פגיעה: A Assay אמין כדי לזהות מבחן הגורמים תיקון Neuromuscular הצומת
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JoVE Journal 神経科学
The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions
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עצב שׁוֹקִיתִי השיטה פגיעה: A Assay אמין כדי לזהות מבחן הגורמים תיקון Neuromuscular הצומת

15,264 Views

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06:01 min

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August 11, 2016

DOI:

10.3791/54186-v

DOI:
10.3791/54186-v

•

06:01 min
August 11, 2016

•

10 Views
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1Carilion Research Institute,Virginia Tech, 2Carilion School of Medicine,Virginia Tech, 3Department of Biological Sciences,Virginia Tech

概要

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We have developed a nerve injury method to reliably examine muscle reinnervation, and thus regeneration of neuromuscular junctions in mice. This technique involves injuring the common fibular nerve via a simple and highly reproducible surgery. Muscle reinnervation in then assessed by whole-mounting the extensor digitorum longus muscle.

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Keyword Extraction:Fibular Nerve InjuryNeuromuscular JunctionsNerve RegenerationPeripheral Nerve InjuryNerve CrushNerve Injury MethodMouse ModelSurgical ProcedureNerve TracingMuscle SeparationNerve ExposureNerve Crush Injury

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Cite this Article

Dalkin, W., Taetzsch, T., Valdez, G. The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions. J. Vis. Exp. (114), e54186, doi:10.3791/54186 (2016).

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