このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
더블 형광 결합<em> 현장에서</em> 마우스 뇌 섹션에서 세 가지 유전자의 발현의 검출을위한 Immunolabelling와 하이브리드
12,765 Views
•
09:23 min
•
March 26, 2016
DOI:
概要
Automatically generated
Localizing gene expression to specific cell types can be challenging due to the lack of specific antibodies. Here we describe a protocol for simultaneous triple detection of gene expression by combining double fluorescence RNA in situ hybridization with immunostaining.
Explore More Videos
Double Fluorescence In Situ HybridizationImmunolabellingGene ExpressionMouse Brain SectionsCell Type LocalizationRNA ProbesFITCDIGTyramideAlkaline PhosphataseFast RedImmunostainingPlasmid DNARNA Polymerase PromotersCDNARestriction EnzymeLinearizationPurificationPhenol-chloroform ExtractionEthanol Precipitation
Read Article
Cite this Article
Jolly, S., Fudge, A., Pringle, N., Richardson, W. D., Li, H. Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections. J. Vis. Exp. (109), e53976, doi:10.3791/53976 (2016).
Copy