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Glycan Node Analysis: A Bottom-up Approach to Glycomics
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化学
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JoVE Journal 化学
Glycan Node Analysis: A Bottom-up Approach to Glycomics

Glycan Node Analysis: A Bottom-up Approach to Glycomics

10,196 Views

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11:36 min

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May 22, 2016

DOI:

10.3791/53961-v

DOI:
10.3791/53961-v

•

11:36 min
May 22, 2016

•

3 Views
, , , ,
1Department of Chemistry & Biochemistry, The Biodesign Institute – Center for Personalized Diagnostics,Arizona State University

概要

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This article presents an enhanced form of a novel bottom-up glycomics technique designed to analyze the pooled compositional profile of glycans in unfractionated biofluids through the chemical breakdown of glycans into their constituent linkage-specific monosaccharides for detection by GC-MS. Potential applications include early detection of cancer and other glycan-affective disorders.

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Zaare, S., Aguilar, J. S., Hu, Y., Ferdosi, S., Borges, C. R. Glycan Node Analysis: A Bottom-up Approach to Glycomics. J. Vis. Exp. (111), e53961, doi:10.3791/53961 (2016).

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