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在脑核糖凝视与原子力显微镜
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JoVE Journal 神経科学
Peering at Brain Polysomes with Atomic Force Microscopy
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在脑核糖凝视与原子力显微镜

8,117 Views

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08:49 min

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March 16, 2016

DOI:

10.3791/53851-v

DOI:
10.3791/53851-v

•

08:49 min
March 16, 2016

•

2 Views
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1Laboratory of Biomolecular Sequence and Structure Analysis for Health,Fondazione Bruno Kessler, 2Institute of Biophysics,CNR Unit at Trento

概要

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While ribosome structure has been extensively characterized, the organization of polysomes is still understudied. To overcome this lack of knowledge, we present here a detailed preparation protocol for accurate imaging of mammalian polysomes by atomic force microscopy (AFM) in air and liquid.

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Cite this Article

Lunelli, L., Bernabò, P., Bolner, A., Vaghi, V., Marchioretto, M., Viero, G. Peering at Brain Polysomes with Atomic Force Microscopy. J. Vis. Exp. (109), e53851, doi:10.3791/53851 (2016).

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