ジャーナル
/
免疫学と感染
/
فحوصات النوعية والكمية للكشف وتوصيف مضادات الميكروبات البروتين
JoVE Journal
免疫学と感染
This content is Free Access.
JoVE Journal 免疫学と感染
Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
Please note that all translations are automatically generated. Click here for the English version.

فحوصات النوعية والكمية للكشف وتوصيف مضادات الميكروبات البروتين

18,126 Views

•

10:50 min

•

April 10, 2016

DOI:

10.3791/53819-v

DOI:
10.3791/53819-v

•

10:50 min
April 10, 2016

•

10 Views
, ,
1Department of Advanced Technology,The MITRE Corporation, 2Center for Biomedical Engineering,Brown University, 3The MITRE Corporation

概要

Automatically generated

Here, we present protocols to rapidly screen and characterize enzymes for antimicrobial activity. The microslide diffusion assay and the dye-release assay utilize target bacterial substrates for qualitative and quantitative enzymatic activity evaluation.

Explore More Videos

Protein AntimicrobialsAntimicrobial ActivityMicroslide Diffusion AssayDye-release AssayBCA Protein AssayAgarose SolutionSodium AzideBacterial SubstrateMcFarland Turbidity StandardSerial DilutionBovine Serum AlbuminPositive Control

Read Article

Cite this Article

Farris, M. H., Ford, K. A., Doyle, R. C. Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials. J. Vis. Exp. (110), e53819, doi:10.3791/53819 (2016).

Download .ris file

Copy

Share Video

.

Copy