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新颖的指标来表征线虫胚胎伸长率<em>秀丽隐杆线虫</em
JoVE Journal
神経科学
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JoVE Journal 神経科学
Novel Metrics to Characterize Embryonic Elongation of the Nematode Caenorhabditis elegans

新颖的指标来表征线虫胚胎伸长率<em>秀丽隐杆线虫</em

DOI:

10:46 min

March 28, 2016

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  • 00:05標題
  • 00:52Characterizing Early Elongation Using 4D (Time Lapse Z-stack) DIC Imaging
  • 03:47Characterizing Late Elongation Using Flow Cytometry
  • 08:23Results: 4D-DIC versus Flow Cytometry for Sizing Nematode Larvae
  • 09:32Conclusion

概要

自動翻訳

Here we detail protocols specifically designed to monitor morphogenic defects that occur during early and late phases of embryonic elongation of the nematode Caenorhabditis elegans. Ultimately, these protocols are designed to identify genes that regulate these phases and to characterize their differential requirements along the antero-posterior axis of the embryo.

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