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A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
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JoVE Journal 発生生物学
A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells

A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells

DOI:
10.3791/53655-v

•

08:34 min

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March 03, 2016

•

, ,
1Institute of Clinical Biochemistry,Hannover Medical School

章

  • 00:05標題
  • 00:34Human ES Cell Differentiation toward the Definitive Endoderm
  • 02:57CXCR4+ Definitive Endoderm (DE) Cell Immunostaining for Flow Cytometric Analysis
  • 04:55CXCR4+ Cell Magnetic Bead Isolation
  • 06:34Results: Representative Changes in Gene/Protein Expression after Human ESC into DE Differentiation
  • 07:59Conclusion

概要

自動翻訳

自動翻訳

Here, we describe a method for the purification of differentiated human embryonic stem cells that are committed towards the definitive endoderm for the improvement of downstream applications and further differentiations.

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Endoderm CellsHuman Embryonic Stem CellsPurificationDifferentiationCell CultureBasement Membrane MatrixROCK InhibitorPrimitive Streak InductionEndoderm InductionSingle-cell Suspension

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