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enChIPによって特定のゲノム領域と関連する分子の同定の単離
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP

enChIPによって特定のゲノム領域と関連する分子の同定の単離

DOI:

09:26 min

January 20, 2016

,

  • 00:05標題
  • 00:41Preparation of Tagged Chromatin
  • 04:16Chromatin Immunoprecipitation
  • 06:41Immunoprecipitated Chromatin Analysis
  • 08:08Results: enChIP to Telomere and the Interferon Regulatory Factor-1 (IRF-1) Promoter Regions
  • 09:00Conclusion

概要

自動翻訳

The identification of molecules associated with specific genomic regions of interest is required to understand the mechanisms of regulation of the functions of these regions. This protocol describes procedures to perform engineered DNA-binding molecule-mediated chromatin imunoprecipitation (enChIP) for identification of proteins and RNAs associated with a specific genomic region.

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