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توصيف الأيض من الاستقطاب M1 M2 الضامة والعظام نخاع المستمدة من طريق في الوقت الحقيقي التحليل خارج الخلية الجريان
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免疫学と感染
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JoVE Journal 免疫学と感染
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
Please note that all translations are automatically generated. Click here for the English version.

توصيف الأيض من الاستقطاب M1 M2 الضامة والعظام نخاع المستمدة من طريق في الوقت الحقيقي التحليل خارج الخلية الجريان

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07:45 min

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November 28, 2015

DOI:

10.3791/53424-v

DOI:
10.3791/53424-v

•

07:45 min
November 28, 2015

•

28 Views
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1Department of Medical Biochemistry, Experimental Vascular Biology,Academic Medical Center, Amsterdam

概要

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Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.

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Macrophage PolarizationM1 MacrophagesM2 MacrophagesMetabolic ReprogrammingGlycolysisOxidative PhosphorylationExtracellular Flux AnalysisOxygen Consumption RateExtracellular Acidification RateMitochondrial ActivitySpare Respiratory Capacity

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Cite this Article

Van den Bossche, J., Baardman, J., de Winther, M. P. Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis. J. Vis. Exp. (105), e53424, doi:10.3791/53424 (2015).

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