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A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
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December 18, 2015
DOI:
1Department of Genetics and Developmental Biology, The Ruth and Bruce Rappaport Faculty of Medicine, Technion,Israel Institute of Technology, 2Department of Ophthalmology,Hillel Yaffe Medical Center, 3Bioimging Center, Biomedical Core Facility, The Ruth and Bruce Rappaport Faculty of Medicine, Technion,Israel Institute of Technology
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In this article we describe the principles for designing and performing a multi-color lineage tracing experiment using R26R-Confetti mice. We provide a specific protocol for tracking corneal epithelial cells which can be modified for other tissues of interest.
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Amitai-Lange, A., Berkowitz, E., Altshuler, A., Dbayat, N., Nasser, W., Suss-Toby, E., Tiosano, B., Shalom-Feuerstein, R. A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice. J. Vis. Exp. (106), e53370, doi:10.3791/53370 (2015).
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