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Quantificazione del colon le mutazioni delle cellule staminali
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Quantification of Colonic Stem Cell Mutations

Quantificazione del colon le mutazioni delle cellule staminali

DOI:

07:53 min

September 25, 2015

,

  • 00:05標題
  • 02:10Preparation of Glucose-6-phosphate Dehydrogenase (G6PD) Staining Mixture
  • 03:56Tissue Section Staining
  • 05:19Determination of Mutation Frequency
  • 06:14Results: Colonic Stem Cell Mutation Frequency as Determined by Analysis of G6PD Mutant Crypts
  • 07:12Conclusion

概要

自動翻訳

We report significant improvements for the reproducible measurement of somatic colonic stem cell mutations after exposure of mice to potential DNA damaging agents.

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