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באמצעות מבודד המיטוכונדריה מכמויות מינימליות של שרירי שלד עכבר למדידות נשימה microplate תפוקה הגבוה
章
- 00:05標題
- 01:30Setup
- 02:53Running the Assay
- 07:45Results: Microplate Based Assay Measures Oxygen Consumption in Skeletal Muscle Mitochodria
- 10:05Conclusion
The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.
