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使用分离线粒体从小鼠骨骼肌进行高通量微孔板呼吸测量最小数量
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
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使用分离线粒体从小鼠骨骼肌进行高通量微孔板呼吸测量最小数量

DOI:
10.3791/53216-v

•

10:39 min

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October 30, 2015

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, , , , ,
1The Department of Human Nutrition, Foods, and Exercise,Virginia Tech, 2The Metabolic Phenotyping Core,Virginia Tech, 3Seahorse Bioscience

章

  • 00:05標題
  • 01:30Setup
  • 02:53Running the Assay
  • 07:45Results: Microplate Based Assay Measures Oxygen Consumption in Skeletal Muscle Mitochodria
  • 10:05Conclusion

概要

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自動翻訳

The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.

タグ

Key Words:Mitochondrial RespirationSkeletal MuscleMouseMicroplateHigh-throughputIsolated MitochondriaRespiratory Measurements

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