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由16S rRNA基因测序和MALDI-TOF MS罕见的细菌病原鉴定
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JoVE Journal 免疫学と感染
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
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由16S rRNA基因测序和MALDI-TOF MS罕见的细菌病原鉴定

16,467 Views

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06:34 min

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July 11, 2016

DOI:

10.3791/53176-v

DOI:
10.3791/53176-v

•

06:34 min
July 11, 2016

•

6 Views
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1Institut für Medizinische Mikrobiologie und Hygiene, Medizinische Fakultät Carl Gustav Carus,TU Dresden, 2Institut für Virologie, Medizinische Fakultät Carl Gustav Carus,TU Dresden

概要

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Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and molecular techniques (16S rRNA gene sequencing) permit the identification of rare bacterial pathogens in routine diagnostics. The goal of this protocol lies in the combination of both techniques which leads to more accurate and reliable data.

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Cite this Article

Schröttner, P., Gunzer, F., Schüppel, J., Rudolph, W. W. Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS. J. Vis. Exp. (113), e53176, doi:10.3791/53176 (2016).

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