Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Markus Axmann; Gerhard J. Sch&#252;tz; Johannes B. Huppa

doi:10.3791/53157

ジャーナル

/

生物工学

/

Medição de TCR-pMHC Binding In Situ Usando a microscopia Ensaio de FRET

JoVE Journal
生物工学
Author Produced

This content is Free Access.

JoVE Journal 生物工学

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Medição de TCR-pMHC Binding In Situ Usando a microscopia Ensaio de FRET

12,022 Views

•

19:05 min

•

October 30, 2015

DOI:

10.3791/53157-v

DOI:

10.3791/53157-v

•

19:05 min

October 30, 2015

•

6 Views

Markus Axmann¹, Gerhard J. Schütz¹, Johannes B. Huppa²

¹Institute for Applied Physics – Biophysics,Vienna University of Technology, ²Institute for Hygiene and Applied Immunology,Medical University of Vienna

概要

This manuscript describes how to conduct (single molecule) Förster Resonance Energy Transfer (FRET)- based assays to measure the binding dynamics between T-cell antigen receptor (TCR) and antigenic peptide-loaded MHC molecules as they occur within the immunological synapse of a T-cell in contact with a functionalized planar supported lipid bilayer.

Explore More Videos

TCR-pMHC Binding FRET-based Microscopy T-cell Antigen Sensitivity Planar Lipid Bilayer Single Molecule FRET Receptor-ligand Interactions

Read Article

Medição de TCR-pMHC Binding<em> In Situ</em> Usando a microscopia Ensaio de FRET

概要

Automatically generated

Explore More Videos