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الميكروية مزدوجة الماسورة والمركزة لقياس خارج الخلية ايون إشارات في أنسجة المخ
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神経科学
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JoVE Journal 神経科学
Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue
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الميكروية مزدوجة الماسورة والمركزة لقياس خارج الخلية ايون إشارات في أنسجة المخ

13,152 Views

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11:08 min

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September 05, 2015

DOI:

10.3791/53058-v

DOI:
10.3791/53058-v

•

11:08 min
September 05, 2015

•

6 Views
, , , ,
1Institute of Neurobiology,Heinrich Heine University Düsseldorf, 2Departments of Physiology and Neuroscience,New York University School of Medicine

概要

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We demonstrate the fabrication, calibration and properties of two types of ion-selective microelectrodes (double-barreled and concentric) for measurement of ion concentrations in brain tissue. These are then used in the mouse hippocampal slice preparation to show that excitatory activity changes both extracellular potassium and sodium concentrations.

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Double-barreled MicroelectrodesConcentric MicroelectrodesExtracellular Ion SignalsPotassiumSodiumHippocampusAcute Brain SliceIon-selective Electrodes

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Haack, N., Durry, S., Kafitz, K. W., Chesler, M., Rose, C. R. Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue. J. Vis. Exp. (103), e53058, doi:10.3791/53058 (2015).

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